Thermo Fisher Scientific CSF1 Monoclonal Antibody (OTI1F8), TrueMAB

Applications

• Western Blot (WB): Tested dilution 1:2,000

Product Specifications

Product Specific Information

• For reconstitution, add 100 µL distilled water to reach a final antibody concentration of ~1 mg/mL.
• For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).

Target Information

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells.
It also induces VEGF secretion by macrophages, mediating mobilization of endothelial progenitor cells and neovascularization.
M-CSF exists as several bioactive isoforms differing in potency and stability. The full-length protein is synthesized as a membrane-spanning form, which can be cleaved and secreted.
It is a disulfide-bonded homodimer, processed into glycoprotein or proteoglycan isoforms modified by chondroitin sulfate.
Binding to its receptor c-Fms (CSF-1R/CD115) triggers receptor dimerization, internalization, and degradation.
Functionally, M-CSF stimulates differentiation of hematopoietic stem cells into monocyte–macrophage lineages.
Human M-CSF shows activity on mouse cells, but mouse CSF does not act on human cells.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.