Takara Taq Antibody for hot-start PCR

Taq Antibody is a monoclonal antibody that binds Taq polymerase and inhibits its activity until reaction temperature is elevated. At that point, the Taq Antibody is denatured and releases its hold on Taq polymerase, allowing DNA synthesis to proceed. The binding of the Taq Antibody to Taq polymerase is the premise of hot-start PCR.

Taq Antibody is a monoclonal antibody that binds Taq polymerase and inhibits its activity until reaction temperature is elevated. At that point, the Taq Antibody is denatured and releases its hold on Taq polymerase, allowing DNA synthesis to proceed. The binding of the Taq Antibody to Taq polymerase is the premise of hot-start PCR.

By preventing Taq polymerase activity until the denaturation step of PCR is underway, nonspecific amplification due to mispriming and/or formation of primer dimers during PCR assembly is prevented. As a result, experimental background is significantly decreased, and reaction specificity is increased.

Taq Antibody can be added to Takara Taq, Takara Ex Taq, or Takara LA Taq polymerase before PCR to lower background, increase specificity, and allow room temperature reaction assembly.

More Less

---

PCR learning center

PCR selection guide

All PCR citations

---

Find answers to your PCR questions

[Primer design](/learning-centers/pcr/faq/primer-design Primer design)

Frequently asked questions about primer design for successful PCR.

[Optimization](/learning-centers/pcr/faq/optimization Optimization)

Frequently asked questions about PCR optimization.

[Troubleshooting](/learning-centers/pcr/faq/troubleshooting Troubleshooting)

Frequently asked questions about troubleshooting your PCR problems.

[Applications and conditions](/learning-centers/pcr/faq/applications-and-conditions Applications and conditions)

Frequently asked questions about general and specific applications for PCR and which polymerases to choose.

[Shipping, storage, and handling](/learning-centers/pcr/faq/shipping-storage-and-handling Shipping, storage, and handling)

Frequently asked questions about shipping, storing, and handling of Takara Bio PCR polymerases.

[Avoid DNA contamination in PCR](/about/bioview-blog/tips-and-troubleshooting/avoid-dna-contamination-in-pcr Avoid DNA contamination in PCR)

There are many ways a PCR experiment can go wrong. Use this guide to prevent common PCR problems.