Takara Xfect RNA Transfection Reagent

Xfect RNA Transfection Reagent is a complete system for highly efficient transfection of mammalian cells with all types of RNA, including messenger RNA, siRNA, single guide RNA (sgRNA), and microRNA. The protocol is simple, requires no optimization, and transfections can be carried out entirely in the presence of serum. While most transfection reagents can be harmful to your cells, the Xfect RNA Transfection Reagent has very low cytotoxicity.

Overview

• Single reagent for transfection with messenger RNA (mRNA), single guide RNA (sgRNA), microRNA (miRNA), and siRNA
• High transfection efficiency and very low cytotoxicity in cell lines and primary cells
• Simple, serum-compatible protocol
• Complete system that includes all components necessary for transfection
• Transfecting mRNA results in faster expression of your protein
• This reagent cannot deliver DNA, but can be combined with Xfect Transfection Reagent for efficient cotransfection of mammalian cells with both DNA and RNA (protocol described in the user manual)

More Information

Applications

• Knockdown of gene expression using siRNA
• Transient transfection with mRNA transcripts
• Knockout of gene expression using CRISPR/Cas9-mediated gene editing

Additional product information

Please see the product`s Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.