Thermo Fisher Scientific DTL Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the N-terminal of human DTL (aa 182-231). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DTL,uniProtId:Q9NZJ0-1,ncbiNodeId:9606,antigenRange:182-231,antigenLength:730,antigenImageFileName:PA5-42842_DTL_Q9NZJ0-1_Rabbit.svg,antigenImageFileNamePDP:PA5-42842_DTL_Q9NZJ0-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2576882

Product Specific Information

Peptide sequence: VNQISGAHNT SDKQTPSKPK KKQNSKGLAP SVDFQQSVTV VLFQDENTLV

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%

Target Information

Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the K+4 motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the Lys-164 monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.