Thermo Fisher Scientific PBR Polyclonal Antibody, FITC
FITC로 표지된 PBR 폴리클로날 항체로, 사람과 랫드 시료에 반응합니다. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합하며, 친화 크로마토그래피로 정제되었습니다. 498/517 nm의 형광 특성을 가지며, -20°C 암소 보관이 필요합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to a unique amino acid sequence on the 18 kDa mitochondrial benzodiazepine receptor protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Mitochondrial peripheral-type benzodiazepine receptor (PBR) is an essential component of the steroidogenic machinery, mediating cholesterol delivery to the inner mitochondrial side chain cleavage cytochrome P-450 upon ligand activation.
PBR consists of three subunits: an isoquinoline binding site, a voltage-dependent anion channel, and an adenine nucleotide carrier. It is conserved from bacteria to humans and is widely expressed in peripheral organs, while in the brain it is primarily localized in glial cells.
Peroxisome proliferator perfluordecanoic acid (PFDA) inhibits Leydig cell steroidogenesis by affecting PBR mRNA stability, reducing PBR expression, cholesterol transport into mitochondria, and subsequent steroid formation.
A cytoplasmic protein, PRAX-1 (peripheral benzodiazepine receptor-associated protein 1), specifically interacts with PBR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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