Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Alexa Fluor 700, eBioscience
Mouse EpCAM(CD326) 인식 G8.8 클론의 Alexa Fluor 700 결합 단일클론 항체. Flow cytometry 및 IHC 등 다양한 응용에 적합. 633–647 nm 레이저로 여기, 723 nm 방출. 암 연구 및 epithelial cell 분석에 유용.
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Thermo Fisher Scientific CD326 (EpCAM) Monoclonal Antibody (G8.8), Alexa Fluor 700, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 8 publications |
| Flow Cytometry (Flow) | 0.25 µg/test | View 59 publications |
| Affinity Purification (AP) | - | View 1 publication |
Product Specifications
| Specification | Details |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 700 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | G8.8 |
| Conjugate | Alexa Fluor™ 700 |
| Excitation / Emission Max | 696 / 719 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848471 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant Violet 711
- Brilliant Violet 786
- eFluor 450
- FITC
- PE
- PE-Cyanine5
- PE-Cyanine7
- PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Custom conjugation available
Product Specific Information
The G8.8 monoclonal antibody reacts with the 40 kDa mouse EpCAM (epithelial cellular adhesion molecule), also known as EGP40, 17-1A antigen, TACSTD1, and CD326. The immunogen used was the TE-71 thymic epithelial cell line. CD326 is expressed on most epithelial cells and is considered a pan-carcinoma antigen mediating calcium-independent, homophilic cell-cell adhesion and potentially functioning as a growth factor receptor. It binds LAIR-1 (CD305) and LAIR-2 (CD306) to inhibit cellular activation and inflammation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on TE-71 cell line; ≤0.25 µg per test (100 µL final volume). Cell number range: 10⁵–10⁸ cells/test. Titrate for optimal performance.
Fluorescence Characteristics:
- Excitation: 633–647 nm
- Emission: 723 nm
- Laser: Red Laser
- Recommended filters: 685 LP mirror, 710/20 band pass filter
Target Information
Ep-CAM (epithelial adhesion molecule, ESA) is a transmembrane glycoprotein (~40 kDa) expressed in epithelial tissues. It functions as a calcium-independent homotypic adhesion molecule, influencing cell cycle, proliferation, and metabolism by inducing genes such as c-myc, cyclin A, and E. Ep-CAM-mediated adhesions negatively regulate cadherin-mediated adhesions, affecting epithelial differentiation and growth. Overexpression is associated with enhanced epithelial proliferation and is highly expressed in human carcinomas, serving as a marker for epithelial lineage tumors. It is localized on basolateral surfaces of simple epithelia and many carcinoma types and helps distinguish adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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