Thermo Fisher Scientific Human Glucocorticoid Receptor (aa 346-367) Synthetic Peptide

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

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Miscellaneous PubMed (Misc)

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View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Class

Synthetic

Type

Peptide

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

purified

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

PEP-001 is a 22 amino acid (amino acids 346-367) synthetic peptide whose sequence is from the amino terminus of the human glucocorticoid receptor (GR). The sequence of this peptide is (amino to carboxy terminus): D-Q-K-P-I-F-N-V-I-P-P-I-P-V-G-S-E-N-W-N-R-C (The C-terminal cysteine has been added to facilitate conjugation and is not a part of the native human GR sequence)

This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and the human GR, catalog # PA1-511A. Using a solution with equal weights per unit volume of peptide and corresponding antibody will yield a solution with a large molar excess of peptide that is able to competitively bind the antibody.

Reconstitute with 0.1 mL of distilled water.

Target Information

Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. Glucocorticoid Receptor can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Glucocorticoid Receptor is expressed in the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. Alternate splicing, the use of at least three different promoters, and alternate translation initiation sites result in several transcript variants encoding the same protein or different isoforms, but the full-length nature of some variants has not been determined. Mutations in the Glucocorticoid Receptor gene are a cause of glucocorticoid resistance, or cortisol, resistance. Studies have shown that glucocorticoid receptor (GR) must be associated with a complex of chaperone proteins for ligand activation. GR binds to known steroids such as dexamethasone with nanomolar affinity

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.