Thermo Fisher Scientific TRIM25 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 190-379 of human TRIM25 (NP_0050732) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRIM25,uniProtId:Q14258-1,ncbiNodeId:9606,antigenRange:190-379,antigenLength:630,antigenImageFileName:PA5-88875_TRIM25_Q14258-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88875_TRIM25_Q14258-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805187

Product Specific Information

Positive Samples: HepG2, A-431, Mouse uterus; Cellular Location: Cytoplasm

Immunogen sequence: SLSQASADLE ATLRHKLTVM YSQINGASRA LDDVRNRQQD VRMTANRKVE QLQQEYTEMK ALLDASETTS TRKIKEEEKR VNSKFDTIYQ ILLKKKSEIQ TLKEEIEQSL TKRDEFEFLE KASKLRGIST KPVYIPEVEL NHKLIKGIHQ STIDLKNELK QCIGRLQEPT PSSGDPGEHD PASTHKSTRP

Target Information

TRIM25, also known as estrogen responsive finger protein (EFP) is a member of the RING finger-B box-coiled coil family and is a downstream target of estrogen receptor a. Mice lacking this gene displayed an underdeveloped uterus and reduced estrogen responsiveness demonstrating that this protein is essential for estrogen dependent proliferation. TRIM25 expression is increased in breast carcinoma where it promotes the growth of breast tumor by functioning as an E3 ubiquitin ligase that targets the negative cell cycle checkpoint 14-3-3s. Recent reports have indicated that TRIM25 E3 ubiquitin ligase is essential for the retinoic-acid-inducible gene 1 (RIG-1) signaling pathway to elicit host antiviral innate immunity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.