Thermo Fisher Scientific DGKQ Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human DGKQ. Recombinant protein control fragment (Product #RP-102422). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DGKQ,uniProtId:P52824-1,ncbiNodeId:9606,antigenRange:498-598,antigenLength:942,antigenImageFileName:PA5-55422_DGKQ_P52824-1_Rabbit.svg,antigenImageFileNamePDP:PA5-55422_DGKQ_P52824-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2640552

Product Specific Information

Immunogen sequence: HVSLFVGGLP PGLSPEEYSS LLHEAGATKA TVVSVSHIYS SQGAVVLDVA CFAEAERLYM LLKDMAVRGR LLTALVLPDL LHAKLPPDSC PLLVFVNPKS G

Highest antigen sequence identity to the following orthologs: Mouse - 82%, Rat - 84%.

Target Information

Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, thereby terminating diacylglycerol signaling, which results in the reduction of protein kinase C activity and cell cycle progression of T lymphocytes. Diacylglycerol kinases are divided into five subtypes, Type I-Type V. DGK-theta is a Type V DGK, and localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12 and HeLa. Nuclear DGK-theta co-localizes with phosphatidylinositol 4, 5-bisphosphate (PIP(2)). DGK-theta is the isoform responsive to alpha-Thrombin stimulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.