Thermo Fisher Scientific PLEKHM1 Monoclonal Antibody (1C9)

Applications

Tested Dilution

Publications

ELISA (ELISA)

1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

1C9

Immunogen

PLEKHM1 (AAH64361, 957 a.a. approximately 1056 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PLEKHM1,uniProtId:Q9Y4G2-1,ncbiNodeId:9606,antigenRange:957-1056,antigenLength:1056,antigenImageFileName:H00009842-M01_PLEKHM1_Q9Y4G2-1_House_mouse.svg,antigenImageFileNamePDP:H00009842-M01_PLEKHM1_Q9Y4G2-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: PHRFSVADLQ QIADGVYEGF LKALIEFASQ HVYHCDLCTQ RGFICQICQH HDIIFPFEFD TTVRCAECKT VFHQSCQAVV KKGCPRCARR RKYQEQNIFA

Target Information

PLEKHM1 is a member of the M family of Pleckstrin homolog domain-containing proteins, a group of proteins containing a RUN domain, two pleckstrin homology domains, and a cysteine-rich domain. It was identified through segregation analysis as a cause of osteopetrosis in humans. PLEKHM1 co-localizes with Rab7 to late endosomal/lysosomal vesicles in HEK293 and osteoclast-like cells, with this co-localization dependent on the prenylation of Rab7. Monocytes from a patient homozygous for a mutated form of PLEKHM1differentiated into osteoclasts normally, but failed to form ruffled borders and showed little evidence of bone resorption when cultured on dentine discs. Another mutation of PLEKHM1 impaired vesicular acidification and increased TRACP secretion in osteoclasts, suggesting that PLEKHM1 has critical roles in endosomal maturation and may be important in osteoclast-osteoblast cross-talk.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.