Thermo Fisher Scientific CD15 Monoclonal Antibody (MCS-1), FITC
인간 CD15 항원에 특이적인 마우스 IgG3 단클론 항체로, FITC 형광 표지되어 유세포분석(Flow Cytometry)에 적합합니다. 세포 응집을 유발하지 않으며, PBS 완충액에 보존되고 4°C 암소 저장이 권장됩니다. Hodgkin병 진단 연구에 활용됩니다.
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Applications
- Flow Cytometry (Flow): 10 µL / 1×10⁶ cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG3 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MCS-1 |
| Immunogen | White blood cells |
| Target Family | SSEA1 (CD15) |
| UniProt ID | P22083-1 |
| Antigen Range | 1–530 |
| Antigen Length | 530 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Purification | Affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075394 |
Product Specific Information
This monoclonal anti-CD15 antibody is an IgG3 fraction which will not agglutinate cells, unlike most IgM antibodies that cause cell aggregation in the cytometer.
Target Information
CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa cells, nervous system cells, and tumor cells.
Variants include sialyl-Lewis x and sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation via calcium-dependent Le(x)-Le(x) interaction, involved in embryo compaction and teratocarcinoma cell autoaggregation.
Sialyl-Le(x) and its isomer sialyl-Le(a) act as selectin ligands. CD15 expression is widely used to confirm the diagnosis of Hodgkin’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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