Thermo Fisher Scientific GATM Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 38-423 of human GATM (NP_0014731?) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GATM,uniProtId:P50440-1,ncbiNodeId:9606,antigenRange:38-423,antigenLength:423,antigenImageFileName:PA5-109756_GATM_P50440-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109756_GATM_P50440-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.98 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855167

Product Specific Information

Immunogen sequence: STQAATASSR NSCAADDKAT EPLPKDCPVS SYNEWDPLEE VIVGRAENAC VPPFTIEVKA NTYEKYWPFY QKQGGHYFPK DHLKKAVAEI EEMCNILKTE GVTVRRPDPI DWSLKYKTPD FESTGLYSAM PRDILIVVGN EIIEAPMAWR SRFFEYRAYR SIIKDYFHRG AKWTTAPKPT MADELYNQDY PIHSVEDRHK LAAQGKFVTT EFEPCFDAAD FIRAGRDIFA QRSQVTNYLG IEWMRRHLAP DYRVHIISFK DPNPMHIDAT FNIIGPGIVL SNPDRPCHQI DLFKKAGWTI ITPPTPIIPD DHPLWMSSKW LSMNVLMLDE KRVMVDANEV PIQKMFEKLG ITTIKVNIRN ANSLGGGFHC WTCDVRRRGT LQSYLD

Target Information

AGAT, also known as glycine amidinotransferase (L-arginine:glycine amidinotransferase), GATM or transamidinase, is a 423 amino acid protein belonging to the amidinotransferase family. Encoded by a gene that maps to human chromosome 15q21.1, AGAT exists as three alternatively spliced isoforms and consists of a homodimer, with equilibrium between monomeric and dimeric forms favoring a monomer subunit structure. AGAT localizes to mitochondrial inner membranes, peripheral membranes and cytoplasm. Biallelically expressed in placenta and fetal tissues, AGAT is also expressed in brain, heart, liver, lung, salivary gland and skeletal muscle tissue, with high expression in kidney. AGAT is elevated in the myocardium during heart failure and is decreased in inter-uterine growth restriction (IUGR)-associated placenta. AGAT catalyzes biosynthesis of guanidinoacetate, the immediate precursor of creatine, which plays a vital role in energy metabolism in muscle tissues. AGAT defects are associated with arginine:glycine amidinotransferase deficiency, an autosomal recessive disorder characterized by developmental delay or regression, mental retardation, severe disturbance of expressive and cognitive speech and severe depletion of creatine/phosphocreatine in brain. AGAT may be linked to embryonic and central nervous system development and may function in heart failure response by elevating local creatine synthesis.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.