Thermo Fisher Scientific MST1-2/STK3-4 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
• Predicted to react with Rat, Zebrafish, and X. laevis based on 100% sequence identity

Target Information

STK3 and STK4 are stress-activated, pro-apoptotic kinases that, following caspase cleavage, translocate to the nucleus to induce chromatin condensation and DNA fragmentation. They are key components of the Hippo signaling pathway, which regulates organ size and tumor suppression by controlling proliferation and apoptosis.

The core Hippo pathway involves a kinase cascade where STK3/MST2 and STK4/MST1, with regulatory protein SAV1, activate LATS1/2 and MOB1. This leads to phosphorylation and inactivation of the YAP1 oncoprotein, preventing its nuclear translocation and regulating genes involved in proliferation, cell death, and migration.

STK3/MST2 and STK4/MST1 repress mature hepatocyte proliferation, prevent activation of oval cells, and inhibit tumor formation. They phosphorylate NKX2-1, NEK2, MOBKL1A, RASSF2, and MOBKL1B, and regulate centrosome disjunction and ESR1 transcriptional activity. They also positively regulate RAF1 activation by suppressing inhibitory phosphorylation on Ser-259.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.