Thermo Fisher Scientific BMP4 Monoclonal Antibody (UMAB42), UltraMAB
BMP4 단백질을 인식하는 UMAB42 단클론 항체로, Western blot, IHC, ICC/IF, ChIP 등 다양한 응용에 적합합니다. 인간 시료 반응성, Mouse IgG1 형식이며, 동결건조 형태로 제공됩니다. 재구성 후 1 mg/mL 농도로 사용 가능하며, PBS 및 8% 트레할로스 완충액에 보관됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500 | |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 | |
| Immunocytochemistry (ICC/IF) | 1:100 | |
| ChIP assay (ChIP) | Assay-Dependent | |
| Peptide Array (Array) | Assay-Dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UMAB42 |
| Immunogen | Recombinant fragment expressed in E. coli corresponding to amino acids 293–408 of human BMP4 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The BMP4 gene encodes a protein belonging to the bone morphogenetic protein family, part of the transforming growth factor-beta (TGF-β) superfamily. Members of this family are growth and differentiation factors originally identified for their ability to induce endochondral osteogenesis.
BMP4 plays a critical role in the initiation of endochondral bone formation in humans. Reduced expression has been linked to bone disorders such as Fibrodysplasia Ossificans Progressiva.
Alternative splicing in the 5′ untranslated region generates three transcript variants encoding the same protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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