Thermo Fisher Scientific BCL6 Monoclonal Antibody (BCL-DWN), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 4 publications

Product Specifications

Additional Formats

• APC
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This BCL-DWN monoclonal antibody reacts with mouse Bcl-6, also known as B-cell lymphoma 6. Bcl-6 is a 79-kDa transcriptional repressor expressed predominantly in germinal center B cells, as well as in differentiating and activated B cells. It is essential for germinal center B cell proliferation and survival, and plays a key role in pre-B cell survival during development.

Bcl-6 is constitutively expressed in diffuse large B cell lymphomas (DLBCLs), Burkitt’s lymphoma, and non-Hodgkin’s lymphoma. It is also found in CD4+ T follicular helper cells and memory T cells, and is known to be antagonized by Blimp-1.

Applications Reported:
Intracellular staining followed by flow cytometric analysis.

Applications Tested:
Validated by flow cytometric analysis of splenocytes from sheep red blood cell-immunized mice. Recommended usage ≤ 0.25 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Titrate for optimal performance.

Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

Bcl-6 proto-oncogene product is a Kruppel-type zinc-finger protein mainly expressed in normal germinal center B cells and related lymphomas. It is involved in chromosome rearrangements at 3q27 in non-Hodgkin’s lymphomas, with rearrangements detected in 33–45% of diffuse large B cell lymphomas. Bcl-6 has been observed immunohistochemically in follicular lymphomas, DLBCL, Burkitt’s lymphomas, and nodular lymphocyte predominant Hodgkin’s disease.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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