Thermo Fisher Scientific MerTK Monoclonal Antibody (DS5MMER), PerCP-eFluor 710, eBioscience
Mouse MerTK 인식 단클론 항체로 PerCP-eFluor 710 형광체 결합. Flow cytometry에 최적화되어 있으며, 세포 표면 MerTK 분석에 사용. 488 nm 청색 레이저로 여기, 710 nm 방출. 고순도 친화 크로마토그래피 정제 및 4°C 암소 보관.
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Thermo Fisher Scientific MerTK Monoclonal Antibody (DS5MMER), PerCP-eFluor 710, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 0.25 µg/test
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Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PerCP-eFluor™ 710, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | DS5MMER |
| Immunogen | FC-tagged full protein expressed in insect cells (MERTK, UniProt ID: Q60805-1, Mouse, amino acids 1–994) |
| Conjugate | PerCP-eFluor™ 710 |
| Excitation / Emission Max | 482 / 708 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688094 |
Additional Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Brilliant UV 395
- Brilliant UV 737
- Brilliant UV 805
- Brilliant Violet 421
- Brilliant Violet 480
- Functional Grade
- PE
- PE-Cyanine7
- Super Bright 436
- Super Bright 600
- Super Bright 645
- Super Bright 702
- Super Bright 780
- Request custom conjugation
Product Specific Information
This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170–210 kDa member of the TAM family of tyrosine kinase receptors (Axl, Tyro3). MerTK is expressed on tissue macrophages and mediates removal of apoptotic cells via Protein S and Gas6 ligands. Activation triggers PI3K/Akt signaling, leading to phagocytosis and suppression of TLR-induced cytokines.
Deficiency causes autoimmunity and inflammation. MerTK is released by metalloproteinases and found in serum and culture medium.
Useful for distinguishing macrophages from dendritic cells; expressed on malignant cells and may contribute to immune evasion.
- Works on paraformaldehyde-fixed cells
- Reported application: Flow cytometric analysis
- Tested on mouse resident peritoneal cells (≤ 0.25 µg/test)
- Recommended titration for optimal performance
- Excitation: 488 nm; Emission: 710 nm; Laser: Blue
- Light-sensitive: protect from light
- Fixation: samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) up to 3 days at 4°C
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
MERTK (cMER) is a tyrosine kinase proto-oncogene involved in retinal pigment epithelium (RPE) phagocytosis and various physiological processes including survival, migration, differentiation, and efferocytosis. Ligand binding induces autophosphorylation and downstream signaling via GRB2, PLCG2, MAPK1/2, FAK/PTK2, and RAC1.
Functions in macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization, and inhibition of TLR-mediated innate immune response through STAT1 activation and SOCS1/3 induction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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