Thermo Fisher Scientific MerTK Monoclonal Antibody (DS5MMER), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific MerTK Monoclonal Antibody (DS5MMER), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.25 µg/test
  View 4 publications

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Brilliant UV 395
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Functional Grade
• PE
• PE-Cyanine7
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation

Product Specific Information

This DS5MMER monoclonal antibody recognizes mouse MerTK, a 170–210 kDa member of the TAM family of tyrosine kinase receptors (Axl, Tyro3). MerTK is expressed on tissue macrophages and mediates removal of apoptotic cells via Protein S and Gas6 ligands. Activation triggers PI3K/Akt signaling, leading to phagocytosis and suppression of TLR-induced cytokines.
Deficiency causes autoimmunity and inflammation. MerTK is released by metalloproteinases and found in serum and culture medium.
Useful for distinguishing macrophages from dendritic cells; expressed on malignant cells and may contribute to immune evasion.

• Works on paraformaldehyde-fixed cells
• Reported application: Flow cytometric analysis
• Tested on mouse resident peritoneal cells (≤ 0.25 µg/test)
• Recommended titration for optimal performance
• Excitation: 488 nm; Emission: 710 nm; Laser: Blue
• Light-sensitive: protect from light
• Fixation: samples can be stored in IC Fixation Buffer (Product #00-8222) or 1-step Fix/Lyse Solution (Product #00-5333) up to 3 days at 4°C
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

MERTK (cMER) is a tyrosine kinase proto-oncogene involved in retinal pigment epithelium (RPE) phagocytosis and various physiological processes including survival, migration, differentiation, and efferocytosis. Ligand binding induces autophosphorylation and downstream signaling via GRB2, PLCG2, MAPK1/2, FAK/PTK2, and RAC1.
Functions in macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization, and inhibition of TLR-mediated innate immune response through STAT1 activation and SOCS1/3 induction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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