Thermo Fisher Scientific Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 0.125 µg/test
Publications: View 9 publications

Product Specifications

Product Specific Information

Description:
The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1). Ly-49 family NK-cell receptors are C-type lectin superfamily members, disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains that bind MHC class I alloantigens.
Ly-49G2 is an inhibitory receptor expressed on subsets of NK cells, NK-T cells (in all strains tested), and a population of memory CD8+ T cells (in C57BL/6 mice). The 4D11 antibody cross-reacts with Ly-49AB6, Ly-49ABALB, Ly-49T129/J inhibitory receptors, and Ly-49LCBA/J activating receptors.
NK-T cells expressing Ly-49A and Ly-49G2 are more frequent in the thymus than in liver, and down-regulation of Ly-49 receptor expression is required for normal NK-T-cell development.

Applications Reported:
This eBio4D11 (4D11) antibody is reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse spleen cells. Use ≤0.125 µg per test (100 µL total volume). Cell number range: 10⁵–10⁸ cells/test. Optimize antibody titration for best performance.

Fluorochrome Information:
PerCP-eFluor® 710 can replace PE-Cy5, PE-Cy5.5, or PerCP-Cy5.5.

• Excitation: 488 nm (blue laser)
• Emission: 710 nm
• Recommended filters: 685 LP dichroic mirror + 710/40 band pass filter (alternative: 695/40 band pass filter)

PerCP-eFluor® 710 conjugated antibodies remain stable when exposed to freshly prepared 2% formaldehyde overnight at 4°C. Evaluate for alternative fixation protocols.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and help prevent NK cells from attacking normal cells while enabling attack on infected or transformed cells with down-regulated class I molecules. These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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