Thermo Fisher Scientific Histone H3 Monoclonal Antibody (865R2)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2 µg/mL

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Immunocytochemistry (ICC/IF)

2 µg/mL

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ChIP assay (ChIP)

-

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Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Mouse, Plant

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

865R2

Immunogen

Recombinant human histone H3 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.2, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10375008

Target Information

Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3 along with four core histone proteins binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Post translationally, histones are modified in a variety of ways to either directly change the chromatin structure or allow for the binding of specific transcription factors. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.