Thermo Fisher Scientific Phospho-Aconitase 1 (Ser138) Polyclonal Antibody
Phospho-Aconitase 1 (Ser138)에 특이적인 Rabbit Polyclonal Antibody로 인산화된 IREB1 검출에 적합. Western blot에서 1:500–1:2,000 희석으로 사용. 인간, 생쥐, 랫트 반응성. 액상 형태, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ACO1 (Accession P21399), corresponding to amino acid residues around phosphorylated Ser138 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816627 |
Product Specific Information
Antibody detects endogenous levels of IREB1 only when phosphorylated at Ser138.
Target Information
Aconitase 1 (ACO1), also known as iron regulatory element binding protein 1 (IREB1), is a cytosolic protein that binds to iron-responsive elements (IREs). It plays a central role in cellular iron homeostasis.
IREs are stem-loop structures found in the 5′ UTR of ferritin mRNA and in the 3′ UTR of transferrin receptor mRNA. Iron-induced binding to IRE results in repression of ferritin translation and inhibition of transferrin receptor mRNA degradation.
Thus, IREB1 regulates iron metabolism and also exhibits aconitase enzymatic activity, grouping it within the aconitase family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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