Thermo Fisher Scientific H3K9me1 Polyclonal Antibody
Rabbit polyclonal antibody targeting mono-methylated lysine 9 of human histone H3.1. Validated for WB, ICC/IF, and ELISA. Supplied as liquid, unconjugated form with antigen affinity purification. Suitable for chromatin and epigenetic research.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide sequence around site of Mono-methyl-Lys (9) derived from Human Histone H3.1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.03 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% Proclin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_3093654 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to change chromatin structure or facilitate transcription factor binding. The N-terminal tail of histone H3 can undergo modifications such as methylation, acetylation, or phosphorylation, influencing cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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