Thermo Fisher Scientific USP5 Monoclonal Antibody (OTI2F5), TrueMAB
USP5 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, IHC, ICC/IF에 적합. HEK293T 세포에서 발현된 full-length human USP5 항원을 사용. Lyophilized 형태로 제공되며, PBS buffer에 trehalose 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2F5 |
| Immunogen | Full length human recombinant protein of human USP5 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
USP5 (ubiquitin carboxyl-terminal hydrolase 5) cleaves linear and branched multiubiquitin polymers with a preference for branched forms.
It participates in disassembly of unanchored Lys-48-linked polyubiquitin and binds linear and Lys-63-linked polyubiquitin with lower affinity.
Knockdown of USP5 leads to accumulation of p53/TP53 and increased transcriptional activity due to competition between unanchored polyubiquitin and ubiquitinated p53/TP53 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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