Thermo Fisher Scientific USP5 Monoclonal Antibody (OTI1F8), TrueMAB
USP5 단백질을 인식하는 Mouse monoclonal 항체로, WB 및 IHC(P) 검증 완료. Human, Dog, Rat 반응성. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 포함. 연구용으로 p53 관련 단백질 분해 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F8 |
| Immunogen | Full length human recombinant protein of human USP5 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
USP5 (Ubiquitin Carboxyl-terminal Hydrolase 5)
USP5 cleaves linear and branched multiubiquitin polymers, showing preference for branched forms. It participates in unanchored Lys-48-linked polyubiquitin disassembly and binds linear and Lys-63-linked polyubiquitin with lower affinity.
Knockdown of USP5 leads to accumulation of p53/TP53 and increased transcriptional activity, as unanchored polyubiquitin competes with ubiquitinated p53/TP53 for proteasomal recognition.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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