Thermo Fisher Scientific Synaptotagmin 1 Polyclonal Antibody
Rabbit polyclonal antibody targeting Synaptotagmin 1, reactive with human, mouse, and rat. Suitable for IHC (paraffin) applications. High purity (>95%) and antigen affinity purified. Supplied in PBS with glycerol; store at 4°C or -20°C.
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Applications
- Immunohistochemistry (Paraffin) (IHC (P)): 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to amino acids 170–220 of Human Synaptotagmin 1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 7.2) with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803002 |
Product Specific Information
Purity is greater than 95% by SDS-PAGE.
Target Information
Synaptotagmin 1 is a synaptic vesicle membrane glycoprotein widely expressed throughout the central nervous system. It functions as a Ca²⁺ sensor regulating exocytosis and neurotransmitter release. Synaptotagmin’s Ca²⁺-mediated binding to SNAP25 is essential for Ca²⁺-dependent membrane triggering. Specific residues within the C2B binding domain influence endocytic rate and vesicle size. Synaptotagmin 1 also regulates synaptic vesicle trafficking at the active zone and binds acidic phospholipids requiring both an acidic head group and diacyl backbone. It interacts with neurexins, syntaxin, and AP2 in a Ca²⁺-independent manner. Dysfunctions are associated with food-borne and infant botulism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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