Thermo Fisher Scientific PCNA Monoclonal Antibody (OTI1H10), TrueMAB

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

PCNA (Proliferating Cell Nuclear Antigen) is essential for DNA replication and participates in DNA excision and mismatch repair pathways. It interacts with CDK inhibitor p21, endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT).
PCNA serves as a marker for proliferating cells and can indicate tumor proliferation status. It is mainly localized in the nucleus and functions as a cofactor of DNA polymerase delta, forming a homotrimer to enhance leading strand synthesis during replication.
Upon DNA damage, PCNA undergoes ubiquitination and participates in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been identified, and pseudogenes exist on chromosome 4 and the X chromosome.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.