Thermo Fisher Scientific SPHK1 Monoclonal Antibody (M540)

Applications

Western Blot (WB)

• Tested Dilution: 1:250

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:200

Product Specifications

Product Specific Information

The antibody detects 42 kDa full-length recombinant human and mouse SK1 proteins, as well as 42, 50, and 60 kDa forms of SK1 in human HeLa cells.

Target Information

SPHK1, along with SPHK2, catalyzes the phosphorylation of sphingosine producing the signaling molecule sphingosine-1-phosphate (S1P).
S1P signals through G-protein coupled receptors and controls diverse cellular processes.
Inhibitors of SPHKs are viewed as potential therapeutics for the treatment of cancer.
SPHK1 catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P), an important signaling molecule with intra- and extracellular functions.
Inside the cell, S1P acts as a signaling molecule similar to other sphingolipid metabolites like ceramide and sphingosine.
S1P has been implicated in regulating cell differentiation, calcium mobilization from intracellular stores, and apoptosis.
The cell surface receptors for S1P are the EDG family of G protein-coupled receptors (S1P Receptors).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.