Thermo Fisher Scientific Histone H2A.X Polyclonal Antibody
Rabbit polyclonal antibody targeting human Histone H2A.X. Validated for IHC, IHC (Paraffin), and ELISA applications. Affinity-purified, unconjugated, liquid form with 0.04 mg/mL concentration. Detects H2AX phosphorylation related to DNA damage re...
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | 1:10–1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| ELISA | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from Human Histone H2AX protein (130–142aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.04 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.03% ProClin 300 |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2914990 |
Product Specific Information
Antibody tested in Indirect ELISA.
Target Information
Histone H2A.X (H2AX) is a member of the histone H2A family, one of the four core histones forming the nucleosome core particle. DNA double-strand breaks (DSBs) trigger phosphorylation of serine 139 at the carboxy terminus of H2AX, producing gamma-H2AX, a marker of DSBs detectable by Western blotting or immunofluorescence. Phosphorylation is mediated by phosphatidylinositol 3-kinases, with ATM, DNA-PK, and ATR implicated in this process. H2A.X consists of 143 amino acids and is synthesized during G1 and S phases. Its mRNA exists in two forms—one polyadenylated (~1600 bases) and one non-polyadenylated (~575 bases). The gene maps to human chromosome 11q23.2–q23.3 and plays a role in nucleosome formation and DNA structure. The H2A.X variant regulates the interaction of MDC1, a DNA repair protein, at damage sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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(PA5-121418_Histone_H2A.X_P16104-1_Rabbit.svg)
(PA5-121418_Histone_H2A.X_P16104-1_Rabbit_PDP.jpeg)
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