Thermo Fisher Scientific ZIPK Polyclonal Antibody
Human ZIPK/DAPK3 인식 폴리클로날 항체로, IHC(P) 및 ICC/IF 실험에 최적화됨. 보존제가 없는 액상 형태이며, Protein A 및 항원 친화 크로마토그래피로 정제됨. 세포 이동성 및 사멸 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the center region of the Human DAPK3/ZIPK |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2787842 |
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human ZIP Kinase/DAPK3.
Target Information
DAPK3 is a serine/threonine kinase functionally linked to cell motility, smooth muscle contractility, and cell death processes.
It is encoded by ZIPK and is a component of desmosomes and the epidermal cornified envelope in keratinocytes. The N-terminal domain interacts with the plasma membrane, while the C-terminus interacts with intermediate filaments.
Through its rod domain, DAPK3 forms complexes with envoplakin and may serve as a link between the cornified envelope, desmosomes, and intermediate filaments.
AKT1/PKB, a protein kinase involved in cell growth and survival signaling, interacts with DAPK3, suggesting a role in AKT1-mediated localization signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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