Invitrogen™

Ambion™ T4 RNA Ligase, cloned, 5 U/μL

Ambion™ T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5-phosphoryl-terminated nucleic acid donor and a 3-hydroxyl-terminated자세히 알아보기

Ambion™ T4 RNA Ligase catalyzes the formation of a phosphodiester linkage between a 5-phosphoryl-terminated nucleic acid donor and a 3-hydroxyl-terminated nucleic acid acceptor. Substrates include RNA, DNA, and oligonucleotides. One tube containing 2,500 U (5 U/ μL) is provided along with 10X Reaction Buffer. The enzyme can be used for tagging the 5ends of mRNA with oligonucleotides for mapping studies (RACE), 3-end labeling RNA molecules, and circularizing RNA and DNA molecules.

Unit Definition:
One unit catalyzes the formation of 1 nmol of [5`-32P]-rA12-18 into a phosphatase-resistant form in 30 min at 37°C.

사양

농도5 U/μL

형식Tube

효소Ligase

Fidelity (Taq 대비)10 X

반응 수250

중합효소SP6 RNA Polymerase

제품라인Ambion™

제품 유형T4 Rna Ligase

수량2,500 units, 5 μL

배송 조건Dry Ice

소스RNA

형태Solution

Unit SizeEach