Invitrogen™

RNase T1 (1 U/μL)

Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion™ RNase T1, RNA-Grade cleaves 3` of자세히 알아보기

Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion™ RNase T1, RNA-Grade cleaves 3` of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites. Supplied in one tube of 200 μL (1U/ μL). RNase T1 can be used to perform boundary experiments to define the minimal RNA sequence required for selectable activities such as protein binding or catalysis. In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.

Unit Definitions:
100 units is the amount of enzyme that yields an increase in absorption at 260 nm of 0.01428 units per min at room temperature using 60 μg/mL yeast total RNA as a substrate.

Accessory Products:
Other RNA-Grade ribonuclease available include RNase A (SKU# AM2274), which cleaves 3` of single-stranded C and U residues. Combinations of the single- and double-stranded specific ribonucleases can provide rapid analysis of the physical structure of an interesting RNA.

사양

농도1 U/μL

형식Tube

효소RNase

Fidelity (Taq 대비)10X

용도(애플리케이션)Real Time PCR (qPCR)

반응 수200

제품라인Ambion™

제품 유형Rnase T1 PCR Kit

수량200 μL

배송 조건Dry Ice

소스RNA

형태Solution

Unit SizeEach