Thermo Fisher Scientific HCG-beta Recombinant Mouse Monoclonal Antibody (rHCGb, 54)
Recombinant Mouse Monoclonal Antibody targeting hCG beta subunit. Validated for IHC (Paraffin). Expressed in HEK293 cells with Protein A/G purification. Supplied as liquid form (200 µg/mL) in PBS with BSA and sodium azide. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rHCGb, 54 |
| Immunogen | Recombinant full-length human hCG beta protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Antigen retrieval: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes.
- Staining of formalin-fixed tissues requires the same heating and cooling procedure as above.
Target Information
hCG beta is the beta chain of human chorionic gonadotropin (hCG), a 40 kDa glycoprotein hormone produced by trophoblastic cells of the placenta starting 10–12 days after conception.
hCG consists of alpha and beta subunits that are non-covalently associated. The alpha subunit is shared with pituitary hormones (FSH, LH, TSH), while the beta subunit provides biological specificity.
During early pregnancy, hCG maintains the corpus luteum, stimulating progesterone production to sustain the endometrium. hCG levels are low in non-pregnant individuals but rise sharply during pregnancy.
Diseases associated with hCG dysfunction include gestational trophoblastic tumor, hydatidiform mole, and recurrent pregnancy-related disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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