Thermo Fisher Scientific AMOTL2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 638-837 of human AMOTL2 (NP_001265612.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:AMOTL2,uniProtId:Q9Y2J4-1,ncbiNodeId:9606,antigenRange:580-779,antigenLength:779,antigenImageFileName:PA5-120481_AMOTL2_Q9Y2J4-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120481_AMOTL2_Q9Y2J4-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.46 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914053

Product Specific Information

Positive test controls include: 293T, U-251MG, HepG2, Mouse liver, Mouse brain.

Immunogen sequence: RQFAMDAAAT AAAQRDTTLI RHSPQPSPSS SFNEGLLTGG HRHQEMESRL KVLHAQILEK DAVIKVLQQR SRRDPGKAIQ GSLRPAKSVP SVFAAAAAGT QGWQGLSSSE RQTADAPARL TTDRAPTEEP VVTAPPAAHA KHGSRDGSTQ TEGPPDSTST CLPPEPDSLL GCSSSQRAAS LDSVATSRVQ DLSDMVEILI

Target Information

Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.