Norgen Streptococcus dysgalactiae TaqMan PCR Detection Kits

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Streptococcus dysgalactiae 검출용 TaqMan PCR 키트로, 유제품 내 병원균 감염 진단 연구에 적합합니다. 프라이머·프로브 세트와 양·음성 대조군 포함, PCR 억제 모니터링 기능 제공. -20°C 보관 시 2년 안정성 유지.

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카테고리: PCR Kits
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Norgen · Norgen Streptococcus dysgalactiae TaqMan PCR Detection Kits

Streptococcus dysgalactiae TaqMan PCR Detection Kits

SKU: TM30750

Features and Benefits

Detection kits for Streptococcus dysgalactiae
Available in TaqMan format for analysis

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the udder is mainly caused by infection of various bacteria. Streptococcus dysgalactiae, a Lancefield group C α-hemolytic gram-positive bacterium, is a common pathogen in subclinical and clinical mastitis causing substantial economic losses in dairy herds. It has been isolated from infected mammary glands and teat injuries and is transmitted primarily during milking. Detection of S. dysgalactiae in other cow tissues such as tonsils, mouth, and vagina, and its occurrence during the non-lactating period, suggest that this organism can also act as an environmental pathogen.

TaqMan PCR Kit Options

Streptococcus dysgalactiae TaqMan PCR Kit (100 reactions)

Ready-to-use format including Master Mix and PCR control
Specific Primer and Probe mix for the pathogen of interest
Positive and negative controls included
Monitors PCR inhibition and validates assay quality

Streptococcus dysgalactiae TaqMan PCR Probe/Primer Set and Controls (100 reactions)

Specific Primer/Probe mix and Positive Control for the target pathogen
Includes nuclease-free water
Compatible with Norgen’s PCR Master Mix (#28007) or user-supplied master mix

For research use only. Not intended for in vitro diagnostics.

Supporting Data

Figure 1. Example of TaqMan PCR Positive Result

Both PCR signals above the baseline from FAM and HEX channels indicate successful PCR.

OCR 정보:

그래프 제목: Amplification
FAM channel (파란색), HEX channel (초록색)
Y축: RFU, X축: Cycles
두 그래프 모두 증폭 곡선을 나타냅니다.

Figure 2. Example of TaqMan PCR Negative Result

No target DNA detected in FAM channel; amplification signal from HEX indicates successful PCR.

OCR 정보:

상단: Amplification – FAM channel
하단: Amplification – HEX channel
Y축: RFU, X축: Cycles
HEX 채널은 약 25사이클 이후 급격히 상승.

Figure 3. Example of TaqMan PCR Inhibition Result

No signal from both FAM and HEX channels detected. Suggested to repeat sample preparation using recommended DNA purification kit.

OCR 정보:

그래프 제목: Amplification
FAM channel (파란색), HEX channel (초록색)
Y축: RFU, X축: Cycles (0–40)
두 채널 모두 신호 없음.

Storage Conditions and Product Stability

All kit components can be stored for 2 years after production without performance reduction.
Store at -20°C upon arrival. Avoid repeated thawing/freezing (>2×) of Master Mix and Positive Control; aliquot if used intermittently.

Kit Components

Component	Cat. TM30750 (100 preps)	Cat. TM30710 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 × 700 μL	–
S. dysgalactiae Primer & Probe Mix	280 μL	280 μL
S. dysgalactiae Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

Documentation

Protocols

(TM30750) Streptococcus dysgalactiae TaqMan PCR Kit - Protocol (100 rxns)

Safety Data Sheets

제품 이미지

Figure 1. Streptococcus dysgalactiae TaqMan PCR Kit
Figure 2. Streptococcus dysgalactiae TaqMan PCR Kit
Figure 3. Streptococcus dysgalactiae TaqMan PCR Kit