Thermo Fisher Scientific Granulysin Monoclonal Antibody (eBioDH2 (DH2)), Alexa Fluor 647, eBioscience

Thermo Fisher Scientific Granulysin Monoclonal Antibody (eBioDH2 (DH2)), Alexa Fluor 647, eBioscience

Applications

• Intracellular staining followed by flow cytometric analysis

Tested Dilution

• 5 µL (0.25 µg)/test

Publications

• View 2 publications

Product Specifications

Additional Formats

• PE Conjugate
• Request custom conjugation

Product Specific Information

The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin belongs to the saposin-like protein (SAPLIP) family. Two proteins, 9 and 15 kDa, are thought to be post-translationally modified versions of the 15 kDa protein with conformational changes.
Granulysin is found in NK and activated T cells, localized to cytolytic granules containing perforin and granzyme. Granulysin and perforin are suspected to act synergistically to kill bacteria and induce apoptosis in nucleated cells.

Applications Reported: Suitable for intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and tested using normal human peripheral blood cells with the Intracellular Fixation & Permeabilization Buffer Set (Product #88-8824-00).
Use at 5 µL (0.25 µg) per test. A test corresponds to staining a cell sample in a final volume of 100 µL. Cell number may range from 10⁵ to 10⁸ cells/test.

Excitation: 633–647 nm
Emission: 668 nm
Laser: Red Laser

Target Information

GNLY is a member of the saposin-like protein (SAPLIP) family, located in cytotoxic granules of T cells and NK cells. It exhibits antimicrobial activity against Mycobacterium tuberculosis and other organisms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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