
Thermo Fisher Scientific PAR1 (F2R) (extracellular) Polyclonal Antibody
PAR1(F2R) 단백질의 세포외 영역을 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF, Flow cytometry에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/BSA 완충액에 동결건조 상태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:200 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 2.5 µg |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61–76 of human PAR-1 (Extracellular, N-terminus) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitution:
Reconstitute with 25 µL, 50 µL, or 0.2 mL of double distilled water (DDW), depending on sample size.
The antibody ships lyophilized at room temperature. Upon arrival, store at -20°C.
After reconstitution, store at 4°C for up to 1 week. For long-term storage, aliquot and keep at -20°C.
Avoid multiple freeze/thaw cycles.
Centrifuge all antibody preparations before use (10,000 × g, 5 min).
Target Information
Thrombin is a serine protease involved in platelet aggregation and blood coagulation. It is generated from prothrombin and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its effects by activating cell surface G-protein coupled receptors, including PAR-1, PAR-2, and PAR-3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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