Thermo Fisher Scientific PAR1 (F2R) (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

2.5 µg

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1, Extracellular, N-terminus if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PAR1,uniProtId:P25116-1,ncbiNodeId:9606,antigenRange:61-76,antigenLength:425,antigenImageFileName:APR-031-200UL_PAR1_P25116-1_Rabbit.svg,antigenImageFileNamePDP:APR-031-200UL_PAR1_P25116-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

Thrombin is a serine protease that is involved in platelet aggregation and blood coagulation. It is cleaved from its precursor, prothrombin, and converts fibrinogen to fibrin in the final step of the clotting cascade. Thrombin mediates its regulatory effects by activating cell surface G-protein coupled receptors which include PAR-1, PAR-2 and PAR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.