Thermo Fisher Scientific ergo-1 Polyclonal Antibody, FITC
C. elegans의 Endogenous-RNAi 관련 Argonaute 단백질을 인식하는 Rabbit Polyclonal Antibody로 FITC 결합 형광 검출 가능. WB, ELISA, IP에 사용 가능하며 고순도 Affinity chromatography로 정제됨. -20°C 암소 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 790–840 on C. elegans endogenous RNAi-deficient Argonaute (ERGO-1) protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | –20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway (PubMed: 17110334, 20133583).
Involved in the 26G RNAi pathway and associates with both unmethylated and methylated 26G-siRNAs, which are 26-nucleotide small interfering RNAs possessing a guanine residue at the 5′ end (PubMed: 22102828, 22548001).
Associated 26G-siRNAs are methylated by the methyltransferase henn-1, which stabilizes the siRNAs (PubMed: 22548001).
Association with 26G-siRNAs is required for the biogenesis of secondary 22G-siRNAs possessing a triphosphorylated guanine residue at the 5′ end (PubMed: 22102828).
May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed: 22102828).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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