Thermo Fisher Scientific ergo-1 Polyclonal Antibody
C. elegans의 Endogenous-RNAi 결핍 Argonaute 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot, ELISA, Immunoprecipitation에 적합하며, 고순도 Affinity chromatography로 정제됨. 액상 형태로 -20°C 보관.
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Thermo Fisher Scientific ergo-1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | C. elegans |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 790–840 on C. elegans Endogenous-RNAi deficient Argonaute protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer (pH 7.4–7.8) with 0.5% BSA, 30% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Argonaute protein required for gene silencing in the endogenous RNA interference (RNAi) pathway (PubMed:17110334, PubMed:20133583).
Involved in the 26G RNAi pathway and associates with both unmethylated and methylated 26G small interfering RNAs (26G-siRNAs), which are a class of 26 nucleotide siRNAs possessing a guanine residue at the 5′-end (PubMed:22102828, PubMed:22548001).
Associated 26G-siRNAs are methylated by the methyltransferase henn-1, which stabilizes the siRNAs (PubMed:22548001).
Association with 26G-siRNAs is required for the biogenesis of secondary 22G-siRNAs (a class of 22 nucleotide siRNAs that possess a triphosphorylated guanine residue at the 5′-end) (PubMed:22102828).
May be involved in passenger strand cleavage of target 26G-siRNAs (PubMed:22102828).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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