Thermo Fisher Scientific 5-Hydroxymethylcytosine (5-hmC) Polyclonal Antibody

Applications

Tested Dilution

Publications

Dot blot (DB)

Assay-dependent

-

Methylated DNA Immunoprecipitation (MeDIP)

1:2,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

The immunogen used to generate this antibody corresponds to 5-HydroxymethylCytosine / 5-hmC

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

7.65 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913087

Product Specific Information

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Information

5-hydroxymethylcytosine (5-hmC) has been recently discovered in mammalian DNA. 5-hmC results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of oxygenases. Initially, the 5-hmC base had been identified in Purkinje neurons, in granule cells and embryonic stem cells where it is present at high levels (up to 0,6% of total nucleotides in Purkinje cells). A recent report indicates that 5-hmC is also abundant in brain tissue, especially in areas that are associated with higher cognitive functions.Early evidence suggests that 5-hmC may represent an intermediate in a new pathway which demethylates DNA, converting 5-mC to cytosine. Although its precise role has still to be shown, recent results indicate, however, that 5-hmC may play important roles distinct from 5-mC. This may open up entirely new perspectives in epigenetic studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.