Thermo Fisher Scientific ZP3 Polyclonal Antibody

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:500–1:1,000

Product Specifications

Product Specific Information

Immunogen sequence:
QPLWLLQGGA SHPETSVQPV LVECQEATLM VMVSKDLFGT GKLIRAADLT LGPEACEPLV SMDTEDVVRF EVGLHECGNS MQVTD

Antigen sequence identity:

• Mouse: 60%
• Rat: 58%

Target Information

The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development.
The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction.

The nascent protein contains:

• N-terminal signal peptide sequence
• Conserved ZP domain
• C-terminal consensus furin cleavage site
• Transmembrane domain

It is hypothesized that furin cleavage releases the mature protein from the plasma membrane for incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage remains controversial based on mouse studies.
A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus; however, sequence and literature review reveal that there is only one full-length ZP3 locus in the human genome.
Another locus, POMZP3, contains a duplication of the last four exons of ZP3 and maps closely to this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.