Thermo Fisher Scientific ATG13 Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• A suggested positive control is rat heart tissue lysate.
• PA5-20999 can be used with blocking peptide PEP-1113.

Target Information

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway, is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components.
This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1.
ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1, and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.