Thermo Fisher Scientific MHC Class I (RT1-A) Monoclonal Antibody (OX18), FITC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

-

Product Specifications

Species Reactivity

Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OX18

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2572463

Product Specific Information

Description: This OX18 monoclonal antibody recognizes a non-polymorphic determinant of rat MHC Class I, which is also known as RT1-A. Rat MHC Class I is expressed on all nucleated cells and erythrocytes.

The OX18 antibody has been reported to have functional activity.

Applications Reported: This OX18 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OX18 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

This protein is a non-polymorphic determinant of rat MHC Class I, which is also known as RT1-A. Rat MHC Class I is expressed on all nucleated cells and erythrocytes.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.