Thermo Fisher Scientific Transferrin Receptor Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

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Immunohistochemistry (IHC)

-

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Immunohistochemistry (Paraffin) (IHC (P))

-

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Immunocytochemistry (ICC/IF)

1:100-1:1,000

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Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 169 and 407 of Human CD71 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Transferrin Receptor (CD71),uniProtId:P02786-1,ncbiNodeId:9606,antigenRange:169-407,antigenLength:760,antigenImageFileName:PA5-27739_Transferrin_Receptor_CD71_P02786-1_Rabbit.svg,antigenImageFileNamePDP:PA5-27739_Transferrin_Receptor_CD71_P02786-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.47 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545215

Product Specific Information

Recommended positive controls: Jurkat, Raji, NCI-H929, K562, HL-60, NIH3T3.

Predicted reactivity: Dog (81%), Cat (86%), Pig (82%), Rhesus Monkey (94%), Bovine (84%), Guinea pig (80%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.