Thermo Fisher Scientific Transferrin Receptor Polyclonal Antibody
Transferrin receptor(CD71)을 인식하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF에 적용 가능. Human과 Mouse 반응성 확인됨. 고순도 항원 친화 크로마토그래피 정제 제품으로 안정적 액상 형태 제공. 철분 대사 및 세포 내 수용체 연구에 적합.
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Thermo Fisher Scientific Transferrin Receptor Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:3,000 | View 1 publication |
| Immunohistochemistry (IHC) | – | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to amino acids 169–407 of Human CD71 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.47 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 20% glycerol, 1% BSA |
| Contains | 0.025% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545215 |
Product Specific Information
- Recommended positive controls: Jurkat, Raji, NCI-H929, K562, HL-60, NIH3T3
- Predicted reactivity: Dog (81%), Cat (86%), Pig (82%), Rhesus Monkey (94%), Bovine (84%), Guinea pig (80%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor.
Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from various conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome, and HIV infection.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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