Thermo Fisher Scientific LIN7A Polyclonal Antibody
Human LIN7A 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, ICC/IF, IP에 사용 가능. 보존제가 포함되지 않아 필요 시 sodium azide 추가 권장. 세포막 단백질 극성 유지 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:300–1:10,000 |
| Immunoprecipitation (IP) | 5–10 µL/mg of lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide corresponding to the center region of the Human LIN7A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A, Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2788721 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
This antibody has specificity for Human LIN7A.
Target Information
LIN7A plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. It forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains.
The tripartite complex composed of LIN7 (LIN7A, LIN7B, or LIN7C), CASK, and APBA1 may couple synaptic vesicle exocytosis to cell adhesion in the brain. LIN7A ensures proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin.
It is required to localize Kir2 channels, GABA transporter (SLC6A12), and EGFR/ERBB1, ERBB2, ERBB3, and ERBB4 to the basolateral membrane of epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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