Thermo Fisher Scientific PKA beta Recombinant Rabbit Monoclonal Antibody (3P5F5)
PKA beta 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, ELISA에 사용 가능. 높은 특이성과 재현성을 제공하며, Human, Mouse, Rat 반응성. 액상 형태로 -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:20,000–1:120,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:200–1:400 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 3P5F5 |
| Immunogen | Synthetic peptide within amino acids 251–351 of human PKA C-beta (PRKACB) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.05% ProClin 300 |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_3094177 |
Product Specific Information
Immunogen sequence:
IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE F
Target Information
The catalytic subunit C-beta of PKA (PRKACB) is a member of the Ser/Thr protein kinase family. The PKA catalytic subunit consists of three gene products: C-alpha, C-beta, and C-gamma, and has been assigned to human chromosome region 1p36.1.
PRKACB is derived from a gene distinct from C-alpha and shows tissue-specific expression. At the amino acid level, C-alpha and C-beta show 93% homology.
The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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