Thermo Fisher Scientific PKA beta Recombinant Rabbit Monoclonal Antibody (3P5F5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:20,000-1:120,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunocytochemistry (ICC/IF)

1:200-1:400

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3P5F5

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 251-351 of human PKA C-beta (PRKACB). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PKA beta,uniProtId:P22694-1,ncbiNodeId:9606,antigenRange:251-351,antigenLength:351,antigenImageFileName:MA5-51456_PKA_beta_P22694-1_Rabbit.svg,antigenImageFileNamePDP:MA5-51456_PKA_beta_P22694-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_3094177

Product Specific Information

Immunogen sequence: IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVS DIKTHKWFAT TDWIAIYQRK VEAPFIPKFR GSGDTSNFDD YEEEDIRVSI TEKCAKEFGE F

Target Information

The catalytic subunit C-beta of PKA (PRKACB) is a member of the Ser/Thr protein kinase family (the PKA catalytic subunit consist of three gene products: C-alpha, C-beta, and C-gamma) and has been assigned to human chromosome region 1p36.1. PRKACB is derived from a gene distinct from C-alpha and shows tissue-specific expression. At the amino acid level C-alpha and C-beta showed 93% homology. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.