Thermo Fisher Scientific PD-L1 (CD274) Polyclonal Antibody
PD-L1(CD274)에 특이적인 Rabbit Polyclonal Antibody로, Western blot과 Flow cytometry에 적합합니다. E.coli 유래 재조합 단백질을 면역원으로 제작되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 동결건조 형태로 제공되며, 재구성 후 500 µg/mL 농도로 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.25–0.5 µg/mL |
| Flow Cytometry (Flow) | 1–3 µg/1×10⁶ cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived human PD-L1 recombinant protein (Position: E45–T290) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 500 µg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 4 mg trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2746089 |
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a B7 family member that binds to the programmed death receptor 1 (PD-1), negatively regulating T-cell receptor (TCR) signaling. PD-L1 ligation reduces TCR-mediated proliferation and cytokine production. It is abundantly expressed in many murine and human cancers and can be upregulated by IFN-γ stimulation, suggesting a key role in tumor immune evasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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