Thermo Fisher Scientific POLE3/CHRAC17 Monoclonal Antibody (PCRP-POLE3-2F10)
인간 POLE3 단백질을 인식하는 Mouse IgG1 단클론 항체로, IHC, ICC/IF, Flow Cytometry, Peptide Array 등 다양한 응용에 적합합니다. 단백질 A/G 정제, 액상 형태, 200 µg/mL 농도로 제공되며, 연구용으로만 사용됩니다.
- 카탈로그번호
- 54107-MSM2-P1
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-POLE3-2F10 |
| Immunogen | Recombinant full-length human POLE3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
Mitochondrial creatine kinase (MtCK) is responsible for transferring high-energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family and exists as two isoenzymes—sarcomeric MtCK and ubiquitous MtCK—encoded by separate genes. MtCK occurs in two oligomeric forms (dimers and octamers), unlike the exclusively dimeric cytosolic isoenzymes. Sarcomeric MtCK shares 80% homology with the coding exons of ubiquitous MtCK. This gene includes motifs common to nuclear genes encoding mitochondrial proteins, potentially essential for coordinated activation during mitochondrial biogenesis. Three transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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