Thermo Fisher Scientific SPT3 Monoclonal Antibody (OTI3D1)

Applications and Tested Dilution

Product Specifications

Target Information

The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from delta sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene.
Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter, allowing TBP to bind to SAGA-dependent promoters.
Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 plays an important role in mating and development pathways in yeast.
At the promoters of some genes including yeast HO, HIS3, and TRP3, Spt3 inhibits TBP binding, resulting in reduced transcription. This repressive effect can be overcome by GCN5, another SAGA complex member, which promotes TBP/TFIIA complex formation via histone acetylation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.