
Thermo Fisher Scientific Normal Mouse Serum
비면역 마우스 혈청으로 비특이적 결합 차단에 적합. IHC, ICC 등 다양한 면역분석에서 블로킹 및 대조용으로 사용. 동결건조 형태로 제공되며 재수화 후 사용 가능. 4°C 보관, 장기 보관 시 분주 후 -20°C 보관 권장.
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Applications
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
| Control (Ctrl) | Assay-dependent |
| Blocking Assay (BLOCK) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Mouse / Ig |
| Class | Control |
| Type | Control |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 50.0 mg/mL |
| Storage buffer | Whole serum/PBS, pH 7.6 |
| Contains | 0.05% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
| RRID | AB_2532172 |
Product Specific Information
For blocking purposes, use a 5% (v/v) solution (1:20 dilution from rehydrated volume). Rehydration according to instructions yields 100% serum.
Restoration and Storage:
Store product at 4°C until opened. Restore with 2.0 mL distilled water. Centrifuge the product if it is not completely clear after standing for 1–2 hours at room temperature. Product may be stored for up to several weeks at 4°C as an undiluted liquid. After dilution, do not use for more than one day. To extend shelf-life, freeze rehydrated undiluted product in small aliquots at –20°C.
Country of Origin: USA
Target Information
Normal sera are non-immune serum samples from goat, human, mouse, rabbit, and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins found in healthy animals.
Normal serum provides sufficient endogenous proteins to saturate and block nonspecific binding sites on fixed samples or assay substrates. When used as an antibody diluent, non-immune serum supplies a native chemical environment for antibodies to function properly without introducing foreign protein components.
Normal serum is frequently used for blocking or saturating generalized binding interactions in immunodetection methods, especially tissue-based assays such as immunohistochemistry (IHC). It is also useful as a control for testing antibody purification methods.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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