Thermo Fisher Scientific POLR2A Monoclonal Antibody (OTI3C8), TrueMAB
POLR2A 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 검증 완료. Human, Mouse, Rat 시료에 반응하며, carrier-free 형태로 제공되어 결합 실험에 적합. 동결건조 형태로 -20°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3C8 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1351–1614 of human POLR2A produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
DNA-directed RNA polymerase II subunit RPB1 (POLR2A) is a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates.
POLR2A is the largest subunit and a catalytic component of RNA polymerase II, responsible for synthesizing mRNA precursors and various non-coding RNAs.
It forms the polymerase active center with the second largest subunit and plays a key role in transcription initiation, elongation, and termination.
Regulation of POLR2A activity is influenced by phosphorylation and acetylation of its C-terminal domain (CTD), which serves as a platform for assembly of transcriptional regulators.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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