Thermo Fisher Scientific Goat anti-Human IgG Fc Cross-Adsorbed Secondary Antibody

Applications

Tested Dilution

Western Blot (WB)

1:2,000 - 1:6,000

Immunohistochemistry (IHC)

Assay-dependent

Immunocytochemistry (ICC/IF)

Assay-dependent

Flow Cytometry (Flow)

Assay-dependent

Immunoprecipitation (IP)

Assay-dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_429669

Target

IgG Fc

Cross Adsorption

Against bovine, horse and mouse serum proteins

Antibody Form

Whole Antibody

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product # 31125 has been successfully used in Western blot, IF, ICC, IHC, IP and FACS applications.

Product # 31125 reacts with the heavy chains of human IgG from only the Fc portion, but not with the light chains or the Fab fragment common to most human immunoglobulins. This antibody does not react against human IgM, IgA or non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with bovine, horse and mouse serum proteins. However, this antibody may cross-react with immunoglobulins from other species.

Store product at 4°C until opened. After opening, dilute only enough antibody for a single day`s use. Store remainder at 4°C under sterile conditions.

Country of origin: USA

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.