Thermo Fisher Scientific Phospho-TrkB (Tyr516) Polyclonal Antibody
Phospho-TrkB (Tyr516) 항체로 인간, 마우스, 랫트 시료에 반응합니다. Western blot, IHC, ELISA에 사용 가능하며, 항원 친화 크로마토그래피로 정제된 rabbit polyclonal antibody입니다. TrkB 신호전달 연구에 적합합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| ELISA | 1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human TrkB around the phosphorylation site of Y516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.5% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607339 |
Product Specific Information
This antibody is predicted to react with human and mouse based on sequence homology.
Target Information
NTRK2 (TRKB) belongs to the neurotrophic factor family essential for the development and maintenance of the mammalian nervous system. NTRK2 acts as the receptor for brain-derived neurotrophic factor (BDNF). Together, NTRK2 and BDNF regulate short-term synaptic functions and long-term potentiation of brain synapses.
TrkB is a tyrosine kinase receptor related to TrkA, primarily expressed in central and peripheral nervous system tissues. BDNF and NT-3 can induce tyrosine phosphorylation of TrkB, with BDNF eliciting a stronger response. Upon neurotrophin binding, TrkB undergoes autophosphorylation and activates MAPK pathway signaling, leading to cell differentiation.
Mutations in the TrkB gene have been linked to obesity and mood disorders. Several splice variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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